Total hack in my opinion but whatever.
100. Mr. Joshua - "Lethal Weapon
99. Beholder - Dungeons and Dragons
98. The Others - "Lost"
97. Francis Buxton - "Pee-wee's Big Adventure"
96. Sephiroth - FFVII
95. Stewie Griffin - "Family Guy"
94. Brainiac - "Superman: The Animated Series"
93. Norman Bates - "Psycho"
92. Dean Wormer - "Animal House"
91. Auric Goldfinger - "Goldfinger"
90. Gentlemen - "Buffy the Vampire Slayer"
89. Bill Lumberg - "Office Space"
88. Frank Booth - "Blue Velvet"
87. Endo - "Lethal Weapon"
86. Mr. Blonde - "Reservoir Dogs"
85. Col. Kurtz - "Apocalypse Now"
84. Deathstroke - DC Comics
83. The French Taunter - "Monty Python and the Holy Grail"
82. Luther - "The Warriors"
81. Angel Eyes - "The Good, The Bad, and The Ugly"
80. Clubber Lang - "Rocky III"
79. Cancer Man - "X-Files"
78. R.J. Fletcher - "UHF"
77. The Flood - Halo: Combat Evolver
76. The Socs - The Outsiders
75. Big Brother - 1984
74. Herr Starr - DC/Vertigo
73. Biff Tannen - "Back to the Future"
72. Tony Montana - "Scarface"
71. Walter Peck - "Ghostbusters"
70. The Balrog - "LOTR: Fellowship"
69. J.R. Ewing - Dallas
68. Megatron - "Transformers"
67. Venom - Marvel Comics
66. Sideshow Bob - "The Simpsons"
65. Kevin - "Sin City"
64. Syndrome - "The Incredibles"
63. Samara - "The Ring"
62. Gollum - "LOTR: Return of the King"
61. Johnny Lawrence - "The Karate Kid"
60. Judge Doom - "Who Framed Roger Rabbit?"
59. Bob - "Twin Peaks"
58. General Zod - "Superman II"
57. Principal Ed Rooney - "Ferris Bueller's Day Off"
56. John Doe - "Se7en"
55. The Overlook Hotel - "The Shining"
54. Leatherface - "Texas Chainsaw Massacre"
53. Cobra Commander - "G.I. Joe"
52. Kid Miracleman - Eclipse Comics
51. Catwoman - DC Comics
50. Max Cady - "Cape Fear"
49. Amanda Woodward - "Melrose Place"
48. Iago - Othello
47. Bill the Butcher - "Gangs of New York"
46. Starscream - "Transformers"
45. Mr. Burns - "The Simpsons"
44. Godzilla - "Godzilla"
43. Serpentor - Marvel Comics
42. Keyser Soze - "The Usual Suspects"
41. Hal 9000 - "2001: A Space Odyssey"
40. Buu - "Dragonball Z"
39. Michael Corleone - "The Godfather: Part II"
38. Dark Phoenix - Marvel Comics
37. Predator - "Predator"
36. Alex Delarge - "A Clockwork Orange"
35. "Rowdy" Roddy Piper - World Wrestling Federation/Entertainment
34. Mayor Richard Wilkins - "Buffy the Vampire Slayer"
33. Tony Soprano - "The Sopranos"
32. Hans Gruber - "Die Hard"
31. Grand Moff Tarkin - "Star Wars"
30. Dracula - "Monster Squad"
29. T-1000 - "Terminator 2: Judgment Day"
28. Jabba The Hutt - "Return of the Jedi"
27. Hillbillies - "Deliverance"
26. Jason Voorhees - "Friday the 13th"
25. Ozymandias - DC Comics
24. Agent Smith - "The Matrix"
23. Darkseid - DC Comics
22. Thanos - Marvel Comics
21. Red Skull - Marvel Comics
20. Khan - "Star Trek"
19. Green Goblin - Marvel Comics
18. Galactus - Marvel Comics
17. Magneto - Marvel Comics
16. The Thing - "John Carpenter's: The Thing"
15. Pennywise - It
14. Freddy Kreuger - "Nightmare on Elm Street"
13. Aliens - "Aliens"
12. Sauron - LOTR
11. Darth Vader - "Star Wars"
10. The Shark - "Jaws"
9. Pinhead - "Hellraiser"
8. Lex Luthor - DC Comics
7. The Borg - "Star Trek: The Next Generation"
6. Hannibal Lecter - "Silence of the Lambs"
5. Zombies - "Dawn of the Dead," 2004
4. Dr. Doom - Marvel Comics
3. Palpatine - "Star Wars"
2. Pazuzu - "The Exorcist"
1. The Joker - DC Comics
Several Antagonists there (It specificly stated Villain, Antagonist a villain be not!)
No Unicroon or even Apocalypse.
Several very odd choices (Out of everything in D&D They chose the Beholder
Why not Tiamat? She was in the Cartoon aswell! And what isn't cooler than a five headed Prismatic dragon G-D?
No Unicron or Apocalypse.
Sephiroth?!
Did I mention the Lack of Unicron?